Structural Organization
of Life
Q No.1. Define discovery of cell and cell theory.
Ans.
In 1665, Robert Hook made a microscope
by combining lenses, called compound microscope and examined a slice of cork
under it. He found small honey comb like chambers which were like small rooms,
so he names these structures as cellulae or cell (small rooms). The cork was
made of bark of oak (Shah Baloot) so he saw cell wall only.
In 1842, Dutrochet, boiled plant
material in nitric acid and then examined under microscope, he found cells. In
1831, Robert Brown discovered nucleus in the cell of orchid. Later the combined
efforts of Schleiden (1838), Theoder
Schwann (1839) and Rudolf Virchow finally gave a cell theory;
The salient features of cell theory
are;
I)
All living organisms are composed of one
or more cells.
II)
The cell is the basic, structural and
functional unit of all organisms.
III)
New cells are formed by the division of
pre-existing cells.
Q No. 2. Differentiate between Light Microscope and Electron
Microscope.
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S. No.
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Light Microscope
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Electron Microscope
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1.
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It Uses visible
light as a source of illumination.
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It uses beam of
electron as a source of illumination.
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2.
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Glass lenses are
used for magnification.
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Electromagnet is
used as lenses.
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3.
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Light microscope can magnify the object up to
1000 times.
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Electron
microscope can magnify the object up to 10,000,000x times.
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4.
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Its resolution
power is just 0.2 m.
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Its resolution
power is 0.2 nm, a thousand times improvement over light microscope.
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5.
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The image of
object can be focused in human eye.
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Screen or
photographic plates are used to focus images.
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Q No. 3. Differentiate between Resolution and Magnification.
Ans.
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S. No.
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Resolution
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Magnification
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1.
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Resolution is the
capacity to separate the adjacent objects.
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Magnification is
the mean of increasing the size of the object.
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2.
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It is maintained
while increasing magnification.
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Resolution is
disturbed while increasing magnification.
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3.
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It improves when
length of illumination becomes shorter.
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It improves with
the focal length of lens.
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Q No. 4. How cell is the structural and functional unit of all
living organisms?
Ans.
Microscopic studies reveal that all living organisms are composed of cells. Therefore,
it is the unit of structure of living organisms. Cells are of different sizes
and shapes so they perform different functions. All basic functional activities
occur in the cell. Therefore, it is also unit of function of all living
organisms.
Q No. 5. Write a note on cell wall.
Ans.
Cell wall is the non-living outermost boundary in plant cells. It is secreted
by protoplasm of plant cell.
Ø In plant cell it is
mainly composed of cellulose and pectin. Cellulose make the fibers which are
arranged in criss cross manner. These fibers are kept in position by a
cementing material called calcium pectate (Pectin).
Ø Bacterial cell-wall
is made up of protein and carbohydrates.
Ø Fungal cell-wall is
made up of fungal cellulose and chitin.
Cell
wall in plants is made up of three layers:
Ø Middle lamella:
Formed between the primary walls of neighboring cells.
Ø Primary wall:
Chemically composed of cellulose and pectin and sometimes lignin.
Ø Secondary wall
Ø Sometimes tertiary
wall
Function: It provides
protection, support and shape to the cell. It is a permeable in nature.
Q No. 6. Write a note on cell membrane.
Ans.
Cell membrane or plasma membrane is an outer layer in all types of cells except
plant and bacterial cell.
Structure: Cell membrane
consists of two layer of Phospho-lipid in which protein molecule floats like
iceberg. This basic structure is called Fluid Mosaic Model presented by Singer
and Nicholson (1972). This basic structure is also found in membranes of
mitochondria, chloroplasts etc.
Function: Cell membrane is
selectively permeable membrane. In many animal cells the cell membrane infolds,
taking in materials in the form of vacuole. This process is called endocytosis.
Q No. 7. Write a note on Nucleus or Karyon.
Ans.
Nucleus was discovered by Robert Brown in 1831. It controls all activities of
cell. It may be spherical or irregular in shape. It is usually present in
center in animal cell while pushed towards cell membrane in plant cell due to a
large vacuole. Nucleus is covered by double nuclear membrane which possesses
large number of nuclear pores. It is filled with jell like nucleoplasm, which
contains nucleolus and thread like structures the chromatin network. Each
thread is called chromosome which is composed of DNA (Deoxyribo nucleic acid)
and protein.
Q No. 8. Write a note on
cytoplasm.
Ans.
Cytoplasm is a fluid portion lying in between cell membrane and nucleus. It
consists of aqueous matrix called cytosol and cytoplasmic organells.
Composition: Cytoplasm is
composed of 90% water and other solutions. Many metabolic reactions like
protein synthesis, glycolysis take place in it.
Some
important cytoplasmic organelles are:
1. Endoplasmic
reticulum 2. Golgi complex 3. Mitochondria 4. Plastids 5. Centrioles
6. Ribosomes 7. Vacuoles
Q No. 9. What are Endoplasmic reticulum and Golgi complex?
Ans.
(Endo=Inside, plasma=protoplasm, reticulum=network). It is the network of
tubules throughout the cytoplasm, which are in contact with plasma membrane and
nuclear membrane. There are two types of endoplasmic reticulum:
1.
Rough Endoplasmic
reticulum: Having ribosomes at outer surface and involve in protein synthesis.
2.
Smooth Endoplasmic
reticulum: It maintains the shape of cell and detoxifies the harmful effects
of drugs.
Golgi
complex were discovered by Camillo Golgi. They are the smooth membranes stacked
into fluid filled sacs containing carbohydrates. Cell secretions are done by
Golgi complex.
Q No. 10. What are Mitochondria and plastids?
Ans.
Mitochondria are bean shaped organelles consisting of double membrane. The
infoldings in inner membrane are called cristae while fluid inside it is called
matrix. Mitochondria contain enzymes which break food for the production of
energy, so they are called power house of cell.
Plastids
are found in cells of higher plants. They contain different types of pigments. Plastids
are of three types on the basis of pigment colour:
I.
Chloroplast: It has green pigment
i.e. Chlorophyll found in leaves and green parts of plants.
II.
Chromoplast: Have coloured
pigment other than green. Found in fruit, flowers, petals and other coloured
parts of plants.
III.
Leucoplast: (Leucos=white or
colourless) found in cells of underground parts of plants. They store food in
the form of starch.
Q No. 11. Write short notes on following:
I.
Centrosome and
centrioles: A round centrosome is present is present near the nucleus in animal
cell. A centrosome contains two centriols, each consists of 9 m
II.
icrotubules. They form fibrous protein
spindle during cell division.
III.
Ribosome: They are
ribonucleic (RNA) acid rich granules. They are attached on the surface of rough
endoplasmic reticulum as well as found as free. They are sites for protein
synthesis hence called protein factories of cell.
IV.
Vacuole: They are the fluid
filled sacs surrounded by a membrane called tonoplast. They store cell sap. In
animal cell they are numerous while in plant cell there is a large vacuole in
center.
Q No.12. What are Prokaryotic and Eukaryotic cells?
Ans.
a. Prokaryotic (Pro=before, Karyon=nucleus) cell does not possess true nucleus,
it means its nuclear membrane is absent. These types of cells are found in
bacteria and cyanobacteria (blue green algae). Such organisms are called
Prokaryotic organisms.
b. Eukaryotic (Eu=true,
Karyon=nucleus) cell possess proper nucleus where nuclear material is enclosed
in proper nuclear membrane. These types of cells are found in plants and
animals and such type of organisms are called eukaryotic organisms.
Q No.13. Differentiate between prokaryotic and eukaryotic cells.
Ans.

Q No.14. What is cell division?
Ans.
New cell after growing to maximum size: may undergo the process of cell
division. During this process, the division of nucleus is called Karyokinesis
(Karyon=nucleus, kinesis=division) and division of cytoplasm is called
Cytokinesis. Thus two daughter cells arise from single division of cell. There
are two main types of cell division 1. Mitosis 2. Meiosis.
Q No. 15. Describe Mitosis and its phases.
Ans.
In Mitosis parent cell divides into two daughter cells in a way that number of
chromosomes in the daughter cells remains the same as in the parent cell.
Karyokinesis can be divided into four phases:
1.
Prophase: During early
prophase chromosomes appear. Each chromosome consists of two chromatids. Two
chromatids in a chromosome are attached to each other at centromere. Nuclear
membrane disappears and centrosome divides into two centrioles, each move to
the opposite pole of the cell and forms the spindle fibres. The centrioles are
absent in plant cell.
2.
Metaphase: During metaphase
each chromosome arranges itself on the equator of the spindle. Each chromosome
is attached to separate spindle fibre by its centromere.
3.
Anaphase: During anaphase
centromere of a chromosome divides and chromatids separate from each other and
move towards opposite poles. In this way each chromatid is now an independent
chromosome. One set of chromatid move towards one pole while other set move
towards other pole.
4.
Telophase: During Telophase
chromatids (now called chromosomes) reach the poles and their movement ceases.
Each pole receives same number of chromosomes as were in the parent cell.
Nuclear membrane is reformed around the each set of chromosomes. Soon the
cytoplasm of cell also divides and two daughter cells arise with same number of
chromosomes as in parent cell.
Q No.16. Describe the significance of Mitosis.
Ans.
Mitosis is responsible for development and growth of organisms by increasing
exact copies of cells. About all kinds of asexual reproduction and vegetative
propagation take pace by mitosis. The production of new somatic cells, such as
blood cells depends upon mitosis. The healing of wounds, repair of wear and
tear within organisms also depend upon mitosis.
Q No.17. Describe Meiosis and its phases.
Ans.
In Meiosis or reduction cell division a parent cell divides into four daughter
cells in a way that number of chromosomes in each daughter cell is reduced to
half of the parent cell (from diploid 2n to haploid n).In animals meiosis
produces gametes (sperm and egg) while spores in plants. Meiosis involves two divisions.
a.
Meiosis I- First meiotic
division or reduction phase.
b.
Meiosis II- Second meiotic
division or meiotic mitotic phase.
a. Meiosis I- First
meiotic division or Reduction phase:
1.
Prophase 1: In this phase
homologous chromosomes come together and form pairs. In each pair of homologous
chromosomes, there are four chromatids, since each chromosome of a pair has
already doubled itself. Homologous chromosomes join to exchange their parts at
certain places. This exchange is called crossing over. During crossing over new
combination of genes result. Nuclear membrane disappears and spindle fibers are
formed.
2.
Metaphase I: During this phase
homologous chromosomes arrange themselves on the equator of spindle.
3.
Anaphase I: Chromosomes of the
homologous pairs now begin to separate and move towards the opposite poles.
4.
Telophase I: At this phase
chromosomes come to rest at the poles. Nuclear membranes are formed around the
each set of chromosomes resulting in formation of two daughter nuclei. Then
cytoplasm also divides thus two daughter cells (having haploid ‘n’ number of
chromosomes) are formed.
b. Meiosis II-
Second meiotic division or Equational division:
1.
Prophase II: Spindles are formed
and nuclear membrane disappears.
2.
Metaphase II: Chromosomes arrange
themselves on the equator.
3.
Anaphase II: Chromatids of
chromosomes separate from each other and migrate to the opposite poles.
4.
Telophase II: Nuclear membrane
reappears around each set of chromatids and now called chromosomes. Then
cytoplasm divides to form two sets of daughter cells.
Q No. 18. Describe the significance of Meiosis.
Ans.
Meiosis plays an important role in keeping chromosome number constant in
species from generation to generation. When haploid sperm fertilizes with
haploid ovum to form zygote, the diploid number (2n) is restored.
Meiosis is responsible for the
difference between individuals of the same species, which was due to crossing
over between homologous chromosomes during Prophase I.
Q No.19. What is a tissue? Describe plant tissues.
Ans.
A tissue is group of common cells which perform common function.
There are two basic types of plant
tissues:
1.
Meristematic
tissues:
This tissue contains cells which have ability to divide. These cells are
smaller in size, thin walled and a nucleus in center. These tissues are present
in root and shoot apex and help in increase of root and shoot length by adding
primary tissue.
2.
Permanent tissues: Cells of these
tissues are formed from meristematic cells. These cells do not divide. The
walls of these cells are thick which maintain their shape.
There are two groups of permanent
tissues:
I.
Simple tissues: These are made up of
one type of cells. It is further be divided into three groups on the basis of
structure:
a.
Parenchyme: Consist of living
cells having intercellular spaces. They are present in soft parts of plants.
These tissues store food.
b.
Collenchyma: Consist of elongated
cells with corners filled with cellulose and pectin. It is present in few
layers under epidermis of herbaceous dicotyledons.
c.
Sclerenchyma: (Scleros=hard) Consist
of very long, narrow, thick walled and lignified cells. They are very hard
because of lignin so provide support to plants. They are found in xylem and
hard coat fruits.
II.
Complex tissues: These are made up
of more than one type of cells. They are mainly of two types:
a.
Xylem: These are
consisting of thick walled elongated tissues. They have sclerenchyma vessels
and fibers which conduct water and salts from soil to leaves and also provide
support.
b.
Phloem: These are
consisting of thin walled elongated living sieve tube like cells, which conduct
food from leaves to all parts of plant.
Xylem and phloem
together form vascular bundle in stem while remain separate from each other in
roots.
Q No. 20. Describe animal tissues.
Ans. There are four important types of animal
tissues:
1.
Epithelial
tissues: Cells of these tissues occur in single layer and
are closely packed together. This tissue forms surface layer under lines of
tubular organs of body. Epithelial tissue occurs in the glands where it is
folded.
2.
Connective
tissue: These tissues provide support to other tissues and
organs bind them together. They have fatty tissues which are soft and
cartilages and bones are hard tissues. Blood is also a special type of
connective tissue with cells suspended in fluid medium.
3.
Muscular
tissues: These are made up of muscle fibers. Each muscle
fiber is a elongated cell which have ability to contract and relax. These tissue
are responsible for movement of body and body parts.
4.
Nervous
tissues: These tissues are made up of cells called neurons or
nerve cells. Nerve cell conduct message in the form of electric currents.
Q No.21. Write a note on amoeba.
Ans. Amoeba is unicellular aquatic organism found in
stagnant water, pools and ponds. Its size is about 0.25 millimeter. It keeps on
changing its shape.
Structure: Cytoplasm of amoeba is
covered by cell membrane. Cytoplasm is differentiated into two parts:
1.
Ectoplasm:
It is an outer portion, it is clear and transparent.
2.
Endoplasm:
It is an inner viscous, translucent and granular part of cytoplasm. It contains
many food vacuoles, contractile vacuole and other cell organelles.
Nucleus is usually present in center but as the
amoeba moves, the nucleus changes its position. Contractile vacuole removes
excess water from the body. It moves and capture food particles by temporary
cytoplasmic projections called pseudopodia (pseudo=false, podia=feet).
QNo.22. Write a note on Brassica
(Brassica campestris).
Ans. Brassica
campestris is the botanical name of mustard (Rai). Its oil is used for
cooking and as hair oil and its leaves are used as vegetable (saag).
This plant consists of:
1.
Vegetative
parts: Those parts which do not directly take part in
sexual reproduction. Such as root, stem, leaves and branches.
2.
Reproductive
parts: Those parts which take part in sexual reproduction.
Such as flowers.
QNo.23: Write a note on flower of
Brassica.
Ans. Brassica plant
bears small yellowish flowers. The arrangement of flowers on stem is called inflorescence. The stalk on which
flower is situated is called pedicle. The tip of the pedicle bears thalamus.
The floral leaves are arranged in four whorls on thalamus.
1.
Calyx:
This is the outer most whorl consists of four free sepals. In young flowers its
colour is light greenish while yellowish in old flowers. It protects inner
parts of flowers from sunlight and rain.
2.
Corolla:
This is second whorl consists of four free yellow petals. Honey bees,
butterflies and other insects are easily attracted due to petals.
3.
Androecium:
This
is third whorl consisting of not-leaf like parts. It has six free stamens which
are the male reproductive part of the flower. In Brassica stamens are arranged in two circles 1. Outer circle has two small stamens and 2. Inner circle has four long stamens. Each stamen has a lower
filament and a upper anther. Each anther has numerous pollen grains. There are
dark green nectaries of small size at the base of androecium, which contain nectar,
which is food of insects.
4.
Gynoecium:
This is the fourth whorl consisting of carpels, which is the female
reproductive part of the plant. In Brassica
the gynoecium is formed by the union of two carpels. Each carpel is divided
into three main parts:
a.
Ovary:
The lower swollen part is called ovary. In ovary many ovules are present, which
are ripen into seeds. The ovary ripens and converted into fruit.
b.
Style:
Above the ovary carpel extends into a thin stalk called style.
c.
Stigma:
Style has a swollen tip which is called stigma.
QNo. 24. Write a note on frog.
Ans. The frog (Rana
tigrina) lives both in water as well as on land. It swims in water and move
by jumping when on land. There are five toes in each foot while the hand has
only four fingers because the thumb is rudimentary. In male frog the first
finger is thicker than the other.
Its
head is directly attached to trunk because of absence of neck. Behind each eye
has a tympanic membrane which helps in hearing. At the tip of snout, frog has
two nostrils. The skin of frog is loose and slippery.
Frogs are found in abundance in rainy
season during which the lay eggs. They hibernate during the winter season by
burying themselves into mud. This phenomenon is called hibernation or winter sleep.
QNo.25. Define respiratory system in
frog.
Ans. Energy required by organisms for life processes
is produced by oxidation of glucose. This process takes place in mitochondria
in cell. In the presence of oxygen in cell, food is oxidized and CO2 is
produced as waste product. This entire process is called respiration.
Respiration is divided into two phases:
1. Gaseous
exchange or Extra-cellular respiration.
2. Cellular
Respiration.
QNo.26. Describe the types of
respiration in frog.
Ans. Frog has three types of respiration.
1.
Pulmonary
Respiration: The gaseous exchange take place by
lungs is called pulmonary respiration.
Frog has two balloon like lungs. Their outer
surface is smooth but their inner surface is folded which increase the area of
gaseous exchange. The lungs are richly supplied with blood vessels. Each lung
has a bronchus. Two bronchus join to form bronchi. Bronchi open into larynx.
Glottis opens into the larynx.
During
respiration air is taken in by the external nostrils. It passes to buccal cavity
through the internal nostril. From here it enters into glottis and passes
through larynx and bronchi finally reach into lungs. From lungs oxygen is taken
by the blood and CO2 is taken out by same route.
2.
Cutaneous
Respiration: Gaseous exchange carried out by the
skin is called cutaneous respiration. Frog uses skin as respiratory organ
during swimming and hibernation. Oxygen diffuses in blood through skin and CO2
diffuses out from blood capillaries in skin.
3.
Buccal
Respiration: The lining of buccal cavity is thin,
moist and richly supplied with capillaries. Here exchange of gases takes place between blood and air.
QNo.27. Describe the circulatory system
in frog.
Ans. Frog has closed type circulatory system in
which blood circulates in closed circuit of blood vessels. It consists of:
I.
Heart
II.
Three kinds of blood
vessels:
a. Arteries:
Which carry blood from heat to other body parts
b. Veins:
Which return blood to heart.
c. Capillaries:
Exchange material between tissues and blood.
QNo.28. Write a note on the heart of frog.
Ans. Heart is a conical organ located in the anterior
region of the body cavity. It is enclosed in a membrane called pericardium. It
contracts and expands which is called heart beat. Frog heart consists of three
chambers.
1. Right
Auricle
2. Left
Auricle
3. Ventricle
The truncus arteriosus
originates from ventral side of ventricle and divides into two branches, each
of which divides into three arteries.
The
oxygenated blood from lungs enters the left auricle through pulmonary veins.
Deoxygenated blood from all parts of body reaches the right auricle. When the
two auricles contract, the blood is pushed into single ventricle. When
ventricle contracts, oxygenated blood is pushed into system arteries and blood
is supplied to all parts of body while deoxygenated blood is pushed into
pulmonary arteries which carry blood to lungs.
QNo. 29. Describe arterial system in
frog.
Ans. A blood vessel which carries blood from heart
to other parts of body is called artery. Arterial system is comprised of three
main components:
1. Pulmocutaneous
Arteries: They supply deoxygenated blood to lungs and skin
where it gives up CO2 and receives oxygen.
2. Carotid
Arteries: These arteries arise from the truncus arteriosus
and supply oxygenated blood to all parts of the head region such as brain,
tongue, head muscles, eyes and ears etc.
3. Systemic
arteries: These vessels carry oxygenated blood to all parts
of body except head and lungs.They fuse together to form aorta, which gives
branches to various parts of body.
QNo.30. Describe the nervous system in
frog.
Ans. The set of organs which control and co-ordinate
all activities of the body is called nervous system. It is composed of two
parts:
1. Central
nervous system: consists of brain and spinal cord
2. Peripheral
nervous system: Consists of nerves, which connect central nervous system with
various parts of body.
The brain consists of following regions:
I.
Most anterior
region is called olfactory lobes.
II.
Behind the
olfactory lobes there are two large outgrowths called cerebral hemispheres.
III.
Behind the
hemispheres two other outgrowths called optic lobes are present.
IV.
The part between
optic lobe and hemispheres is known as diencephalon.
V.
The last part of
brain consists of cerebellum and medulla oblongata.
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